Composite

Part:BBa_K575025

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0034) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
    Illegal AgeI site found at 1494
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830


[edit]
Categories
Parameters
n/aLasP/30/G + CP/34/LasR